Cargando…

RESCUE: a validated Nanopore pipeline to classify bacteria through long-read, 16S-ITS-23S rRNA sequencing

Despite the advent of third-generation sequencing technologies, modern bacterial ecology studies still use Illumina to sequence small (~400 bp) hypervariable regions of the 16S rRNA SSU for phylogenetic classification. By sequencing a larger region of the rRNA gene operons, the limitations and biase...

Descripción completa

Detalles Bibliográficos
Autores principales: Petrone, Joseph R., Rios Glusberger, Paula, George, Christian D., Milletich, Patricia L., Ahrens, Angelica P., Roesch, Luiz Fernando Wurdig, Triplett, Eric W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10402275/
https://www.ncbi.nlm.nih.gov/pubmed/37547696
http://dx.doi.org/10.3389/fmicb.2023.1201064