High-yield ligation-free assembly of DNA constructs with nucleosome positioning sequence repeats for single-molecule manipulation assays

Force and torque spectroscopy have provided unprecedented insights into the mechanical properties, conformational transitions, and dynamics of DNA and DNA–protein complexes, notably nucleosomes. Reliable single-molecule manipulation measurements require, however, specific and stable attachment chemi...

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Detalles Bibliográficos
Autores principales: Lin, Yi-Yun, Brouns, Tine, Kolbeck, Pauline J., Vanderlinden, Willem, Lipfert, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2023
Materias:
Methods and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10404619/
https://www.ncbi.nlm.nih.gov/pubmed/37257819
http://dx.doi.org/10.1016/j.jbc.2023.104874

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10404619/
https://www.ncbi.nlm.nih.gov/pubmed/37257819
http://dx.doi.org/10.1016/j.jbc.2023.104874

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