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Improved spectral resolution of [(13)C,(1)H]-HSQC spectra of aromatic amino acid residues in proteins produced by cell-free synthesis from inexpensive (13)C-labelled precursors
Cell-free protein synthesis using eCells allows production of amino acids from inexpensive (13)C-labelled precursors. We show that the metabolic pathway converting pyruvate, glucose and erythrose into aromatic amino acids is maintained in eCells. Judicious choice of (13)C-labelled starting material...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Netherlands
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406723/ https://www.ncbi.nlm.nih.gov/pubmed/37338652 http://dx.doi.org/10.1007/s10858-023-00420-9 |