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Improved spectral resolution of [(13)C,(1)H]-HSQC spectra of aromatic amino acid residues in proteins produced by cell-free synthesis from inexpensive (13)C-labelled precursors

Cell-free protein synthesis using eCells allows production of amino acids from inexpensive (13)C-labelled precursors. We show that the metabolic pathway converting pyruvate, glucose and erythrose into aromatic amino acids is maintained in eCells. Judicious choice of (13)C-labelled starting material...

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Detalles Bibliográficos
Autores principales: Van Raad, Damian, Huber, Thomas, Otting, Gottfried
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10406723/
https://www.ncbi.nlm.nih.gov/pubmed/37338652
http://dx.doi.org/10.1007/s10858-023-00420-9