Analyzing the topology of N-linked glycans by PNGase F accessibility assay

While N-glycans are synthesized in the lumens, some of them reach the cytosolic side of membranes through retro-translocation independent of endoplasmic-reticulum-associated degradation. Here, we present a protocol to measure the topology of N-glycans in a transmembrane protein, based on the princip...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Jingcheng, Ye, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407232/
https://www.ncbi.nlm.nih.gov/pubmed/37516975
http://dx.doi.org/10.1016/j.xpro.2023.102458

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407232/
https://www.ncbi.nlm.nih.gov/pubmed/37516975
http://dx.doi.org/10.1016/j.xpro.2023.102458

Ejemplares similares