Cargando…
Analyzing the topology of N-linked glycans by PNGase F accessibility assay
While N-glycans are synthesized in the lumens, some of them reach the cytosolic side of membranes through retro-translocation independent of endoplasmic-reticulum-associated degradation. Here, we present a protocol to measure the topology of N-glycans in a transmembrane protein, based on the princip...
| Autores principales: | , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407232/ https://www.ncbi.nlm.nih.gov/pubmed/37516975 http://dx.doi.org/10.1016/j.xpro.2023.102458 |