On-bead purification and nanodisc reconstitution of human chemokine receptor complexes for structural and biophysical studies

Chemokine receptors, a subfamily of G-protein-coupled receptors (GPCRs), are responsible for cell migration during physiological processes as well as in diseases like inflammation and cancers. Here, we present a protocol for solubilizing, purifying, and reconstituting complexes of chemokine receptor...

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Detalles Bibliográficos
Autores principales: Gu, Siyi, Huang, Mian, Handel, Tracy M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407235/
https://www.ncbi.nlm.nih.gov/pubmed/37516969
http://dx.doi.org/10.1016/j.xpro.2023.102460

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407235/
https://www.ncbi.nlm.nih.gov/pubmed/37516969
http://dx.doi.org/10.1016/j.xpro.2023.102460

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