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On-bead purification and nanodisc reconstitution of human chemokine receptor complexes for structural and biophysical studies
Chemokine receptors, a subfamily of G-protein-coupled receptors (GPCRs), are responsible for cell migration during physiological processes as well as in diseases like inflammation and cancers. Here, we present a protocol for solubilizing, purifying, and reconstituting complexes of chemokine receptor...
Autores principales: | Gu, Siyi, Huang, Mian, Handel, Tracy M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407235/ https://www.ncbi.nlm.nih.gov/pubmed/37516969 http://dx.doi.org/10.1016/j.xpro.2023.102460 |
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