CRISPR generation of CSF1R-G795A human microglia for robust microglia replacement in a chimeric mouse model

Chimeric mouse models have recently been developed to study human microglia in vivo. However, widespread engraftment of donor microglia within the adult brain has been challenging. Here, we present a protocol to introduce the G795A point mutation using CRISPR-Cas9 into the CSF1R locus of human pluri...

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Detalles Bibliográficos
Autores principales: Chadarevian, Jean Paul, Davtyan, Hayk, Lombroso, Sonia I., Bennett, F. Chris, Blurton-Jones, Mathew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407259/
https://www.ncbi.nlm.nih.gov/pubmed/37516973
http://dx.doi.org/10.1016/j.xpro.2023.102490

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407259/
https://www.ncbi.nlm.nih.gov/pubmed/37516973
http://dx.doi.org/10.1016/j.xpro.2023.102490

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