Determining DNA–Protein Binding Affinities and Specificities from Crude Lysates Using a Combined SILAC/TMT Labeling Strategy

[Image: see text] In recent years, quantitative mass spectrometry-based interaction proteomics technology has proven very useful in identifying specific DNA–protein interactions using single pull-downs from crude lysates. Here, we applied a SILAC/TMT-based higher-order multiplexing approach to devel...

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Detalles Bibliográficos
Autores principales: Gräwe, Cathrin, Hernandez-Quiles, Miguel, Jansen, Pascal W. T. C., Brimmers, Annika, Vermeulen, Michiel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407929/
https://www.ncbi.nlm.nih.gov/pubmed/37466164
http://dx.doi.org/10.1021/acs.jproteome.3c00248

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407929/
https://www.ncbi.nlm.nih.gov/pubmed/37466164
http://dx.doi.org/10.1021/acs.jproteome.3c00248

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