Cargando…

Determining DNA–Protein Binding Affinities and Specificities from Crude Lysates Using a Combined SILAC/TMT Labeling Strategy

[Image: see text] In recent years, quantitative mass spectrometry-based interaction proteomics technology has proven very useful in identifying specific DNA–protein interactions using single pull-downs from crude lysates. Here, we applied a SILAC/TMT-based higher-order multiplexing approach to devel...

Descripción completa

Detalles Bibliográficos
Autores principales:	Gräwe, Cathrin, Hernandez-Quiles, Miguel, Jansen, Pascal W. T. C., Brimmers, Annika, Vermeulen, Michiel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2023
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407929/ https://www.ncbi.nlm.nih.gov/pubmed/37466164 http://dx.doi.org/10.1021/acs.jproteome.3c00248

Ejemplares similares

Quantification of cellular protein and redox imbalance using SILAC-iodoTMT methodology
por: Vajrychova, Marie, et al.
Publicado: (2019)

Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometry
por: Makowski, Matthew M., et al.
Publicado: (2018)

A SILAC-Based Screen for Methyl-CpG Binding Proteins Identifies RBP-J as a DNA Methylation and Sequence-Specific Binding Protein
por: Bartels, Stefanie J. J., et al.
Publicado: (2011)

DeLTa-Seq: direct-lysate targeted RNA-Seq from crude tissue lysate
por: Kashima, Makoto, et al.
Publicado: (2022)

Data from SILAC-based quantitative analysis of lysates from mouse microglial cells treated with Withaferin A (WA)
por: Narayan, Malathi, et al.
Publicado: (2016)

Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent Capture on Suspension Bead Arrays
por: Huovinen, Tuomas, et al.
Publicado: (2021)

Comparing SILAC- and Stable Isotope Dimethyl-Labeling Approaches for Quantitative Proteomics
por: Lau, Ho-Tak, et al.
Publicado: (2014)

Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates
por: Rautio, Jari, et al.
Publicado: (2003)

TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers
por: Dai, Jiaqi, et al.
Publicado: (2020)

Plant SILAC: Stable-Isotope Labelling with Amino Acids of Arabidopsis Seedlings for Quantitative Proteomics
por: Lewandowska, Dominika, et al.
Publicado: (2013)

Monitoring mitochondrial translation by pulse SILAC
por: Imami, Koshi, et al.
Publicado: (2023)

Cancer vaccines based on whole-tumor lysate or neoepitopes with validated HLA binding outperform those with predicted HLA-binding affinity
por: Fritah, Hajer, et al.
Publicado: (2023)

Status of complete proteome analysis by mass spectrometry: SILAC labeled yeast as a model system
por: de Godoy, Lyris MF, et al.
Publicado: (2006)

Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling
por: Ariosa-Morejon, Yoanna, et al.
Publicado: (2021)

Protocol for high-throughput semi-automated label-free- or TMT-based phosphoproteome profiling
por: Koenig, Claire, et al.
Publicado: (2023)

Cross‐linking mass spectrometry reveals the structural topology of peripheral NuRD subunits relative to the core complex
por: Spruijt, Cornelia G., et al.
Publicado: (2020)

SILAC-iPAC: A quantitative method for distinguishing genuine from non-specific components of protein complexes by parallel affinity capture
por: Rees, Johanna S., et al.
Publicado: (2015)

Analysis of the Membrane Proteome of Ciprofloxacin-Resistant Macrophages by Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)
por: Caceres, Nancy E., et al.
Publicado: (2013)

The necessity of others: Entrepreneurial self-efficacy, TMT collective efficacy, CEO-TMT interface, and entrepreneurial orientation
por: Peng, Xiaobao, et al.
Publicado: (2023)

SILAC-based quantification of changes in protein tyrosine phosphorylation induced by Interleukin-2 (IL-2) and IL-15 in T-lymphocytes
por: Osinalde, Nerea, et al.
Publicado: (2015)

MSstatsTMT: Statistical Detection of Differentially Abundant Proteins in Experiments with Isobaric Labeling and Multiple Mixtures
por: Huang, Ting, et al.
Publicado: (2020)

SILAC kinase screen identifies potential MASTL substrates
por: Marzec, Kamila A., et al.
Publicado: (2022)

Fluorine NMR Spectroscopy Enables to Quantify the Affinity Between DNA and Proteins in Cell Lysate
por: Welte, Hannah, et al.
Publicado: (2021)

Diversity of Active States in TMT Opsins
por: Sakai, Kazumi, et al.
Publicado: (2015)

The Impact of Resource Bricolage on Entrepreneurial Orientation in Start-ups: The Moderating Roles of TMT Heterogeneity and TMT Behavioral Integration
por: Xiaobao, Peng, et al.
Publicado: (2022)

A Genetic Engineering Solution to the “Arginine Conversion Problem” in Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
por: Bicho, Claudia C., et al.
Publicado: (2010)

A combination of SILAC and nucleotide acyl phosphate labelling reveals unexpected targets of the Rsk inhibitor BI-D1870
por: Edgar, Alexander J., et al.
Publicado: (2014)

MS-analysis of SILAC-labeled MYC-driven B lymphoma cells overexpressing miR-17-19b
por: Mihailovich, Marija, et al.
Publicado: (2016)

An Enhanced In Vivo Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Model for Quantification of Drug Metabolism Enzymes
por: MacLeod, A. Kenneth, et al.
Publicado: (2015)

Dataset of differential lipid raft and global proteomes of SILAC-labeled cystic fibrosis cells upon TNF -α stimulation
por: Chhuon, C., et al.
Publicado: (2016)

Mass spectrometry–based relative quantification of proteins in precatalytic and catalytically active spliceosomes by metabolic labeling (SILAC), chemical labeling (iTRAQ), and label-free spectral count
por: Schmidt, Carla, et al.
Publicado: (2014)

proteiNorm – A User-Friendly Tool for Normalization and Analysis of TMT and Label-Free Protein Quantification
por: Graw, Stefan, et al.
Publicado: (2020)

Phenotypic analysis combined with tandem mass tags (TMT) labeling reveal the heterogeneity of strawberry stolon buds
por: Guan, Ling, et al.
Publicado: (2019)

Shotgun and TMT-Labeled Proteomic Analysis of the Ovarian Proteins of an Insect Vector, Aedes aegypti (Diptera: Culicidae)
por: Geiser, Dawn L, et al.
Publicado: (2022)

Assessment of TMT Labeling Efficiency in Large-Scale Quantitative Proteomics: The Critical Effect of Sample pH
por: Hutchinson-Bunch, Chelsea, et al.
Publicado: (2021)

Nonaplex PCR using Cliffhanger primers to identify diarrhoeagenic Escherichia coli from crude lysates of human faecal samples
por: Schneider, Uffe Vest, et al.
Publicado: (2018)

Secretome Analysis of Skeletal Myogenesis Using SILAC and Shotgun Proteomics
por: Chan, C. Y. X'avia, et al.
Publicado: (2011)

Triple SILAC to Determine Stimulus Specific Interactions in the Wnt Pathway
por: Hilger, Maximiliane, et al.
Publicado: (2011)

Combination of SILAC and in situ biotinylation to detect specific protein interacions
por: Blumert, C, et al.
Publicado: (2009)

Hepatic SILAC proteomic data from PANDER transgenic model
por: Athanason, Mark G., et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_osiu42hil9eur6sa0t3hov23an