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Cytosolic Ca(2+) gradients and mitochondrial Ca(2+) uptake in resting muscle fibers: A model analysis

Calcium ions (Ca(2+)) enter mitochondria via the mitochondrial Ca(2+) uniporter, driven by electrical and concentration gradients. In this regard, transgenic mouse models, such as calsequestrin knockout (CSQ-KO) mice, with higher mitochondrial Ca(2+) concentrations ([Ca(2+)](mito)), should display h...

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Autores principales: Marcucci, Lorenzo, Michelucci, Antonio, Reggiani, Carlo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10412765/
https://www.ncbi.nlm.nih.gov/pubmed/37576797
http://dx.doi.org/10.1016/j.bpr.2023.100117
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author Marcucci, Lorenzo
Michelucci, Antonio
Reggiani, Carlo
author_facet Marcucci, Lorenzo
Michelucci, Antonio
Reggiani, Carlo
author_sort Marcucci, Lorenzo
collection PubMed
description Calcium ions (Ca(2+)) enter mitochondria via the mitochondrial Ca(2+) uniporter, driven by electrical and concentration gradients. In this regard, transgenic mouse models, such as calsequestrin knockout (CSQ-KO) mice, with higher mitochondrial Ca(2+) concentrations ([Ca(2+)](mito)), should display higher cytosolic Ca(2+) concentrations ([Ca(2+)](cyto)). However, repeated measurements of [Ca(2+)](cyto) in quiescent CSQ-KO fibers never showed a difference between WT and CSQ-KO. Starting from the consideration that fluorescent Ca(2+) probes (Fura-2 and Indo-1) measure averaged global cytosolic concentrations, in this report we explored the role of local Ca(2+) concentrations (i.e., Ca(2+) microdomains) in regulating mitochondrial Ca(2+) in resting cells, using a multicompartmental diffusional Ca(2+) model. Progressively including the inward and outward fluxes of sarcoplasmic reticulum (SR), extracellular space, and mitochondria, we explored their contribution to the local Ca(2+) distribution within the cell. The model predicts Ca(2+) concentration gradients with hot spots or microdomains even at rest, minor but similar to those of evoked Ca(2+) release. Due to their specific localization close to Ca(2+) release units (CRU), mitochondria could take up Ca(2+) directly from high-concentration microdomains, thus sensibly raising [Ca(2+)](mito), despite minor, possibly undetectable, modifications of the average [Ca(2+)](cyto).
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spelling pubmed-104127652023-08-11 Cytosolic Ca(2+) gradients and mitochondrial Ca(2+) uptake in resting muscle fibers: A model analysis Marcucci, Lorenzo Michelucci, Antonio Reggiani, Carlo Biophys Rep (N Y) Report Calcium ions (Ca(2+)) enter mitochondria via the mitochondrial Ca(2+) uniporter, driven by electrical and concentration gradients. In this regard, transgenic mouse models, such as calsequestrin knockout (CSQ-KO) mice, with higher mitochondrial Ca(2+) concentrations ([Ca(2+)](mito)), should display higher cytosolic Ca(2+) concentrations ([Ca(2+)](cyto)). However, repeated measurements of [Ca(2+)](cyto) in quiescent CSQ-KO fibers never showed a difference between WT and CSQ-KO. Starting from the consideration that fluorescent Ca(2+) probes (Fura-2 and Indo-1) measure averaged global cytosolic concentrations, in this report we explored the role of local Ca(2+) concentrations (i.e., Ca(2+) microdomains) in regulating mitochondrial Ca(2+) in resting cells, using a multicompartmental diffusional Ca(2+) model. Progressively including the inward and outward fluxes of sarcoplasmic reticulum (SR), extracellular space, and mitochondria, we explored their contribution to the local Ca(2+) distribution within the cell. The model predicts Ca(2+) concentration gradients with hot spots or microdomains even at rest, minor but similar to those of evoked Ca(2+) release. Due to their specific localization close to Ca(2+) release units (CRU), mitochondria could take up Ca(2+) directly from high-concentration microdomains, thus sensibly raising [Ca(2+)](mito), despite minor, possibly undetectable, modifications of the average [Ca(2+)](cyto). Elsevier 2023-07-17 /pmc/articles/PMC10412765/ /pubmed/37576797 http://dx.doi.org/10.1016/j.bpr.2023.100117 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Report
Marcucci, Lorenzo
Michelucci, Antonio
Reggiani, Carlo
Cytosolic Ca(2+) gradients and mitochondrial Ca(2+) uptake in resting muscle fibers: A model analysis
title Cytosolic Ca(2+) gradients and mitochondrial Ca(2+) uptake in resting muscle fibers: A model analysis
title_full Cytosolic Ca(2+) gradients and mitochondrial Ca(2+) uptake in resting muscle fibers: A model analysis
title_fullStr Cytosolic Ca(2+) gradients and mitochondrial Ca(2+) uptake in resting muscle fibers: A model analysis
title_full_unstemmed Cytosolic Ca(2+) gradients and mitochondrial Ca(2+) uptake in resting muscle fibers: A model analysis
title_short Cytosolic Ca(2+) gradients and mitochondrial Ca(2+) uptake in resting muscle fibers: A model analysis
title_sort cytosolic ca(2+) gradients and mitochondrial ca(2+) uptake in resting muscle fibers: a model analysis
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10412765/
https://www.ncbi.nlm.nih.gov/pubmed/37576797
http://dx.doi.org/10.1016/j.bpr.2023.100117
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