Cargando…

Models for prediction of single base primer extension efficiency from position and type of single mismatch in primer-template duplex

Amplification and specificity of polymerase chain reaction (PCR) are affected by the position and type of primer-template mismatches (MMs) as well as various conditions of reaction. In this study, multiple linear regression (MLR) models and artificial neural network (ANN) models were developed for t...

Descripción completa

Detalles Bibliográficos
Autores principales: Ri, Myong-Rim, Kang, Jin-Sok, Ri, Myong-Ryong, U, Song Nam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10412835/
https://www.ncbi.nlm.nih.gov/pubmed/37576225
http://dx.doi.org/10.1016/j.heliyon.2023.e18829