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Models for prediction of single base primer extension efficiency from position and type of single mismatch in primer-template duplex
Amplification and specificity of polymerase chain reaction (PCR) are affected by the position and type of primer-template mismatches (MMs) as well as various conditions of reaction. In this study, multiple linear regression (MLR) models and artificial neural network (ANN) models were developed for t...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10412835/ https://www.ncbi.nlm.nih.gov/pubmed/37576225 http://dx.doi.org/10.1016/j.heliyon.2023.e18829 |