A micro-flow, high-pH, reversed-phase peptide fractionation and collection system for targeted and in-depth proteomics of low-abundance proteins in limiting samples

We present a method and a simple system for high-pH RP-LC peptide fractionation of small sample amounts (30–60 µg), at micro-flow rates with micro-liter fraction collection using ammonium bicarbonate as an optimized buffer for system stability and robustness. The method is applicable to targeted mas...

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Detalles Bibliográficos
Autores principales: Zurawska, Marta, Basik, Mark, Aguilar-Mahecha, Adriana, Dadlez, Michal, Domanski, Dominik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Biochemistry, Genetics and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10413349/
https://www.ncbi.nlm.nih.gov/pubmed/37577163
http://dx.doi.org/10.1016/j.mex.2023.102306

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10413349/
https://www.ncbi.nlm.nih.gov/pubmed/37577163
http://dx.doi.org/10.1016/j.mex.2023.102306

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