Protocol for the High-quality Plasmid Isolation from Different Recalcitrant Bacterial Species: Agrobacterium spp., Rhizobium sp., and Bacillus thuringiensis

High yield of good quality plasmid DNA from gram -ve bacteria (Agrobacterium tumefaciens, A. rhizogenes, and Rhizobium sp.) and gram +ve bacterium (Bacillus thuringiensis) is difficult. The widely used plasmid extraction kits for Escherichia coli yield a low quantity of poor-quality plasmid DNA from...

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Detalles Bibliográficos
Autores principales: Kodackattumannil, Preshobha, Sasi, Shina, Krishnan, Saranya, Lekshmi, Geetha, Kottackal, Martin, Amiri, Khaled M. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415192/
https://www.ncbi.nlm.nih.gov/pubmed/37575390
http://dx.doi.org/10.21769/BioProtoc.4788

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415192/
https://www.ncbi.nlm.nih.gov/pubmed/37575390
http://dx.doi.org/10.21769/BioProtoc.4788

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