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Protocol for the High-quality Plasmid Isolation from Different Recalcitrant Bacterial Species: Agrobacterium spp., Rhizobium sp., and Bacillus thuringiensis
High yield of good quality plasmid DNA from gram -ve bacteria (Agrobacterium tumefaciens, A. rhizogenes, and Rhizobium sp.) and gram +ve bacterium (Bacillus thuringiensis) is difficult. The widely used plasmid extraction kits for Escherichia coli yield a low quantity of poor-quality plasmid DNA from...
Autores principales: | Kodackattumannil, Preshobha, Sasi, Shina, Krishnan, Saranya, Lekshmi, Geetha, Kottackal, Martin, Amiri, Khaled M. A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bio-Protocol
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415192/ https://www.ncbi.nlm.nih.gov/pubmed/37575390 http://dx.doi.org/10.21769/BioProtoc.4788 |
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