Fluorescent PCR–based Screening Methods for Precise Knock-in of Small DNA Fragments and Point Mutations in Zebrafish

Generation of zebrafish (Danio rerio) models with targeted insertion of epitope tags and point mutations is highly desirable for functional genomics and disease modeling studies. Currently, CRISPR/Cas9-mediated knock-in is the method of choice for insertion of exogeneous sequences by providing a rep...

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Detalles Bibliográficos
Autores principales: Carrington, Blake, Sood, Raman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415208/
https://www.ncbi.nlm.nih.gov/pubmed/37575394
http://dx.doi.org/10.21769/BioProtoc.4732

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415208/
https://www.ncbi.nlm.nih.gov/pubmed/37575394
http://dx.doi.org/10.21769/BioProtoc.4732

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