Cargando…

High resolution cryo-EM and crystallographic snapshots of the actinobacterial two-in-one 2-oxoglutarate dehydrogenase

Actinobacteria possess unique ways to regulate the oxoglutarate metabolic node. Contrary to most organisms in which three enzymes compose the 2-oxoglutarate dehydrogenase complex (ODH), actinobacteria rely on a two-in-one protein (OdhA) in which both the oxidative decarboxylation and succinyl transf...

Descripción completa

Detalles Bibliográficos
Autores principales:	Yang, Lu, Wagner, Tristan, Mechaly, Ariel, Boyko, Alexandra, Bruch, Eduardo M., Megrian, Daniela, Gubellini, Francesca, Alzari, Pedro M., Bellinzoni, Marco
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415282/ https://www.ncbi.nlm.nih.gov/pubmed/37563123 http://dx.doi.org/10.1038/s41467-023-40253-6

Ejemplares similares

A snapshot of cryo‐EM
por: Skiniotis, Georgios
Publicado: (2016)

Crystallographic and spectroscopic snapshots reveal a dehydrogenase in action
por: Huo, Lu, et al.
Publicado: (2015)

Microtubule structure by cryo-EM: snapshots of dynamic instability
por: Manka, Szymon W., et al.
Publicado: (2018)

Selective Inhibition of 2-Oxoglutarate and 2-Oxoadipate Dehydrogenases by the Phosphonate Analogs of Their 2-Oxo Acid Substrates
por: Artiukhov, Artem V., et al.
Publicado: (2021)

Energy landscapes from cryo-EM snapshots: a benchmarking study
por: Dsouza, Raison, et al.
Publicado: (2023)

Local heterogeneity analysis of crystallographic and cryo-EM maps using shell-approximation
por: Lunin, Vladimir Y., et al.
Publicado: (2023)

Snapshots of the first-step self-splicing of Tetrahymena ribozyme revealed by cryo-EM
por: Zhang, Xiaojing, et al.
Publicado: (2023)

Snapshots of the second-step self-splicing of Tetrahymena ribozyme revealed by cryo-EM
por: Li, Shanshan, et al.
Publicado: (2023)

The cryoEM structure of cytochrome bd from C. glutamicum provides novel insights into structural properties of actinobacterial terminal oxidases
por: Grund, Tamara N., et al.
Publicado: (2023)

Crystallographic snapshot of cellulose synthesis and membrane translocation
por: Morgan, Jacob L.W., et al.
Publicado: (2012)

The Local Resolution of Cryo-EM Density Maps
por: Kucukelbir, Alp, et al.
Publicado: (2013)

The cryo-EM resolution revolution and transcription complexes
por: Hanske, Jonas, et al.
Publicado: (2018)

Single-particle cryo-EM: beyond the resolution
por: Armache, Jean-Paul, et al.
Publicado: (2019)

ABHD11 maintains 2-oxoglutarate metabolism by preserving functional lipoylation of the 2-oxoglutarate dehydrogenase complex
por: Bailey, Peter S. J., et al.
Publicado: (2020)

Cryo-EM snapshots of mycobacterial arabinosyltransferase complex EmbB(2)-AcpM(2)
por: Zhang, Lu, et al.
Publicado: (2020)

Detailed kinetics and regulation of mammalian 2-oxoglutarate dehydrogenase
por: Qi, Feng, et al.
Publicado: (2011)

AlphaFold2 and CryoEM: Revisiting CryoEM modeling in near-atomic resolution density maps
por: Hryc, Corey F., et al.
Publicado: (2022)

Cryo‐EM targets in CASP14
por: Cragnolini, Tristan, et al.
Publicado: (2021)

Oxoglutarate dehydrogenase complex controls glutamate-mediated neuronal death
por: Weidinger, Adelheid, et al.
Publicado: (2023)

Characteristics of the GlnH and GlnX Signal Transduction Proteins Controlling PknG-Mediated Phosphorylation of OdhI and 2-Oxoglutarate Dehydrogenase Activity in Corynebacterium glutamicum
por: Sundermeyer, Lea, et al.
Publicado: (2022)

Cryo-EM snapshots of a native lysate provide structural insights into a metabolon-embedded transacetylase reaction
por: Tüting, Christian, et al.
Publicado: (2021)

Crystallographic Snapshots of Cyanide- and Water-Bound C-Clusters from Bifunctional Carbon Monoxide Dehydrogenase/Acetyl-CoA Synthase(,)
por: Kung, Yan, et al.
Publicado: (2009)

High-resolution cryo-EM proteasome structures in drug development
por: Morris, Edward P., et al.
Publicado: (2017)

High-resolution cryo-EM reconstructions in the presence of substantial aberrations
por: Bromberg, Raquel, et al.
Publicado: (2020)

Automatic local resolution-based sharpening of cryo-EM maps
por: Ramírez-Aportela, Erney, et al.
Publicado: (2019)

Topaz-Denoise: general deep denoising models for cryoEM and cryoET
por: Bepler, Tristan, et al.
Publicado: (2020)

CryoDRGN: Reconstruction of heterogeneous cryo-EM structures using neural networks
por: Zhong, Ellen D., et al.
Publicado: (2021)

Structural Basis for the Binding of Allosteric Activators Leucine and ADP to Mammalian Glutamate Dehydrogenase
por: Aleshin, Vasily A., et al.
Publicado: (2022)

A step-by-step protocol for capturing conformational snapshots of ligand gated ion channels by single-particle cryo-EM
por: Zhang, Kaihua, et al.
Publicado: (2022)

Crystallographic snapshots of a B(12)-dependent radical SAM methyltransferase
por: Fyfe, Cameron D., et al.
Publicado: (2022)

Estimating loop length from CryoEM images at medium resolutions
por: McKnight, Andrew, et al.
Publicado: (2013)

On the explicit use of experimental images in high resolution cryo-EM reﬁnement
por: Cherfils, Jacqueline, et al.
Publicado: (2019)

Microfluidic protein isolation and sample preparation for high-resolution cryo-EM
por: Schmidli, Claudio, et al.
Publicado: (2019)

Reliable cryo-EM resolution estimation with modified Fourier shell correlation
por: Penczek, Pawel A.
Publicado: (2020)

Dynamics in the murine norovirus capsid revealed by high-resolution cryo-EM
por: Snowden, Joseph S., et al.
Publicado: (2020)

Measuring local-directional resolution and local anisotropy in cryo-EM maps
por: Vilas, Jose Luis, et al.
Publicado: (2020)

Interpretation of medium resolution cryoEM maps of multi-protein complexes
por: Casañal, Ana, et al.
Publicado: (2019)

Principal component analysis is limited to low-resolution analysis in cryoEM
por: Sorzano, Carlos Oscar S., et al.
Publicado: (2021)

Measuring the effects of ice thickness on resolution in single particle cryo-EM
por: Neselu, Kasahun, et al.
Publicado: (2023)

Uniform thin ice on ultraflat graphene for high-resolution cryo-EM
por: Zheng, Liming, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_6ebt85la9tjs41snugbjvmloao