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Protocol to assess receptor-ligand binding in C. elegans using adapted thermal shift assays
The Caenorhabditis elegans genome encodes a greatly expanded number of nuclear hormone receptors, many of which remain orphaned. Here, we present a protocol to assess ligand-receptor binding in C. elegans using an adapted cellular thermal shift assay and isothermal dose response. We describe steps f...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415787/ https://www.ncbi.nlm.nih.gov/pubmed/37527042 http://dx.doi.org/10.1016/j.xpro.2023.102477 |