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Protocol to assess receptor-ligand binding in C. elegans using adapted thermal shift assays

The Caenorhabditis elegans genome encodes a greatly expanded number of nuclear hormone receptors, many of which remain orphaned. Here, we present a protocol to assess ligand-receptor binding in C. elegans using an adapted cellular thermal shift assay and isothermal dose response. We describe steps f...

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Detalles Bibliográficos
Autores principales: Tse, Samantha Y., Pukkila-Worley, Read
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415787/
https://www.ncbi.nlm.nih.gov/pubmed/37527042
http://dx.doi.org/10.1016/j.xpro.2023.102477

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