Cargando…
Protocol to assess receptor-ligand binding in C. elegans using adapted thermal shift assays
The Caenorhabditis elegans genome encodes a greatly expanded number of nuclear hormone receptors, many of which remain orphaned. Here, we present a protocol to assess ligand-receptor binding in C. elegans using an adapted cellular thermal shift assay and isothermal dose response. We describe steps f...
Autores principales: | Tse, Samantha Y., Pukkila-Worley, Read |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415787/ https://www.ncbi.nlm.nih.gov/pubmed/37527042 http://dx.doi.org/10.1016/j.xpro.2023.102477 |
Ejemplares similares
-
Surveillance Immunity: An Emerging Paradigm of Innate Defense Activation in Caenorhabditis elegans
por: Pukkila-Worley, Read
Publicado: (2016) -
Immunometabolism in Caenorhabditis elegans
por: Anderson, Sarah M., et al.
Publicado: (2020) -
Candida albicans Infection of Caenorhabditis elegans Induces Antifungal Immune Defenses
por: Pukkila-Worley, Read, et al.
Publicado: (2011) -
Transcriptional suppression of sphingolipid catabolism controls pathogen resistance in C. elegans
por: Nasrallah, Mohamad A., et al.
Publicado: (2023) -
Protocols for treating C. elegans with pharmacological agents, osmoles, and salts for imaging and behavioral assays
por: Wang, Lei, et al.
Publicado: (2023)