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DNA-free high-quality RNA extraction from 39 difficult-to-extract plant species (representing seasonal tissues and tissue types) of 32 families, and its validation for downstream molecular applications

BACKGROUND: High-purity RNA serves as the basic requirement for downstream molecular analysis of plant species, especially the differential expression of genes to various biotic and abiotic stimuli. But, the extraction of high-quality RNA is usually difficult from plants rich in polysaccharides and...

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Detalles Bibliográficos
Autores principales: Sasi, Shina, Krishnan, Saranya, Kodackattumannil, Preshobha, Shamisi, Aysha AL, Aldarmaki, Maitha, Lekshmi, Geetha, Kottackal, Martin, Amiri, Khaled M. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10416385/
https://www.ncbi.nlm.nih.gov/pubmed/37568159
http://dx.doi.org/10.1186/s13007-023-01063-5