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Optimisation of Sample Preparation from Primary Mouse Tissue to Maintain RNA Integrity for Methods Examining Translational Control

SIMPLE SUMMARY: The rate by which the ribosome decodes an mRNA determines the final protein output of the mRNA. This can vary greatly between different mRNAs. Methods which allow accurate determination of translation rates of specific mRNAs require conditions that preserve native mRNA–ribosome inter...

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Detalles Bibliográficos
Autores principales: Munro, June, Gillen, Sarah L., Mitchell, Louise, Laing, Sarah, Karim, Saadia A., Rink, Curtis J., Waldron, Joseph A., Bushell, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10417042/
https://www.ncbi.nlm.nih.gov/pubmed/37568801
http://dx.doi.org/10.3390/cancers15153985