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The evolution of borneol repellency in culicine mosquitoes

Insects have developed remarkable adaptations to effectively interact with plant secondary metabolites and utilize them as cues to identify suitable hosts. Consequently, humans have used aromatic plants for centuries to repel mosquitoes. The repellent effects of plant volatile compounds are mediated...

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Autores principales: Vainer, Yuri, Wang, Yinliang, Huff, Robert M., Ghaninia, Majid, Coutinho-Abreu, Iliano V., Sar-Shalom, Evyatar, Ruiz, Carlos, Perets, Dor, Yakir, Esther, Rajamanickam, Dhivya, Warburg, Alon, Papathanos, Philippos, Akbari, Omar S., Ignell, Rickard, Riffell, Jeff A., Pitts, R. Jason, Bohbot, Jonathan D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10418152/
https://www.ncbi.nlm.nih.gov/pubmed/37577635
http://dx.doi.org/10.1101/2023.08.01.548337
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author Vainer, Yuri
Wang, Yinliang
Huff, Robert M.
Ghaninia, Majid
Coutinho-Abreu, Iliano V.
Sar-Shalom, Evyatar
Ruiz, Carlos
Perets, Dor
Yakir, Esther
Rajamanickam, Dhivya
Warburg, Alon
Papathanos, Philippos
Akbari, Omar S.
Ignell, Rickard
Riffell, Jeff A.
Pitts, R. Jason
Bohbot, Jonathan D.
author_facet Vainer, Yuri
Wang, Yinliang
Huff, Robert M.
Ghaninia, Majid
Coutinho-Abreu, Iliano V.
Sar-Shalom, Evyatar
Ruiz, Carlos
Perets, Dor
Yakir, Esther
Rajamanickam, Dhivya
Warburg, Alon
Papathanos, Philippos
Akbari, Omar S.
Ignell, Rickard
Riffell, Jeff A.
Pitts, R. Jason
Bohbot, Jonathan D.
author_sort Vainer, Yuri
collection PubMed
description Insects have developed remarkable adaptations to effectively interact with plant secondary metabolites and utilize them as cues to identify suitable hosts. Consequently, humans have used aromatic plants for centuries to repel mosquitoes. The repellent effects of plant volatile compounds are mediated through olfactory structures present in the antennae, and maxillary palps of mosquitoes. Mosquito maxillary palps contain capitate-peg sensilla, which house three olfactory sensory neurons, of which two are mainly tuned to either carbon dioxide or octenol - two animal host odorants. However, the third neuron, which expresses the OR49 receptor, has remained without a known ecologically-relevant odorant since its initial discovery. In this study, we used odorant mixtures and terpenoid-rich Cannabis essential oils to investigate the activation of OR49. Our results demonstrate that two monoterpenoids, borneol and camphor, selectively activate OR49, and OR9-expressing neurons, as well as the MD3 glomerulus in the antennal lobe. We confirm that borneol repels female mosquitoes, and knocking out the gene encoding the OR49 receptor suppresses the response of the corresponding olfactory sensory neuron. Importantly, this molecular mechanism of action is conserved across culicine mosquito species, underscoring its significance in their olfactory systems.
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spelling pubmed-104181522023-08-12 The evolution of borneol repellency in culicine mosquitoes Vainer, Yuri Wang, Yinliang Huff, Robert M. Ghaninia, Majid Coutinho-Abreu, Iliano V. Sar-Shalom, Evyatar Ruiz, Carlos Perets, Dor Yakir, Esther Rajamanickam, Dhivya Warburg, Alon Papathanos, Philippos Akbari, Omar S. Ignell, Rickard Riffell, Jeff A. Pitts, R. Jason Bohbot, Jonathan D. bioRxiv Article Insects have developed remarkable adaptations to effectively interact with plant secondary metabolites and utilize them as cues to identify suitable hosts. Consequently, humans have used aromatic plants for centuries to repel mosquitoes. The repellent effects of plant volatile compounds are mediated through olfactory structures present in the antennae, and maxillary palps of mosquitoes. Mosquito maxillary palps contain capitate-peg sensilla, which house three olfactory sensory neurons, of which two are mainly tuned to either carbon dioxide or octenol - two animal host odorants. However, the third neuron, which expresses the OR49 receptor, has remained without a known ecologically-relevant odorant since its initial discovery. In this study, we used odorant mixtures and terpenoid-rich Cannabis essential oils to investigate the activation of OR49. Our results demonstrate that two monoterpenoids, borneol and camphor, selectively activate OR49, and OR9-expressing neurons, as well as the MD3 glomerulus in the antennal lobe. We confirm that borneol repels female mosquitoes, and knocking out the gene encoding the OR49 receptor suppresses the response of the corresponding olfactory sensory neuron. Importantly, this molecular mechanism of action is conserved across culicine mosquito species, underscoring its significance in their olfactory systems. Cold Spring Harbor Laboratory 2023-08-04 /pmc/articles/PMC10418152/ /pubmed/37577635 http://dx.doi.org/10.1101/2023.08.01.548337 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Vainer, Yuri
Wang, Yinliang
Huff, Robert M.
Ghaninia, Majid
Coutinho-Abreu, Iliano V.
Sar-Shalom, Evyatar
Ruiz, Carlos
Perets, Dor
Yakir, Esther
Rajamanickam, Dhivya
Warburg, Alon
Papathanos, Philippos
Akbari, Omar S.
Ignell, Rickard
Riffell, Jeff A.
Pitts, R. Jason
Bohbot, Jonathan D.
The evolution of borneol repellency in culicine mosquitoes
title The evolution of borneol repellency in culicine mosquitoes
title_full The evolution of borneol repellency in culicine mosquitoes
title_fullStr The evolution of borneol repellency in culicine mosquitoes
title_full_unstemmed The evolution of borneol repellency in culicine mosquitoes
title_short The evolution of borneol repellency in culicine mosquitoes
title_sort evolution of borneol repellency in culicine mosquitoes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10418152/
https://www.ncbi.nlm.nih.gov/pubmed/37577635
http://dx.doi.org/10.1101/2023.08.01.548337
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