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The evolution of borneol repellency in culicine mosquitoes
Insects have developed remarkable adaptations to effectively interact with plant secondary metabolites and utilize them as cues to identify suitable hosts. Consequently, humans have used aromatic plants for centuries to repel mosquitoes. The repellent effects of plant volatile compounds are mediated...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10418152/ https://www.ncbi.nlm.nih.gov/pubmed/37577635 http://dx.doi.org/10.1101/2023.08.01.548337 |
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author | Vainer, Yuri Wang, Yinliang Huff, Robert M. Ghaninia, Majid Coutinho-Abreu, Iliano V. Sar-Shalom, Evyatar Ruiz, Carlos Perets, Dor Yakir, Esther Rajamanickam, Dhivya Warburg, Alon Papathanos, Philippos Akbari, Omar S. Ignell, Rickard Riffell, Jeff A. Pitts, R. Jason Bohbot, Jonathan D. |
author_facet | Vainer, Yuri Wang, Yinliang Huff, Robert M. Ghaninia, Majid Coutinho-Abreu, Iliano V. Sar-Shalom, Evyatar Ruiz, Carlos Perets, Dor Yakir, Esther Rajamanickam, Dhivya Warburg, Alon Papathanos, Philippos Akbari, Omar S. Ignell, Rickard Riffell, Jeff A. Pitts, R. Jason Bohbot, Jonathan D. |
author_sort | Vainer, Yuri |
collection | PubMed |
description | Insects have developed remarkable adaptations to effectively interact with plant secondary metabolites and utilize them as cues to identify suitable hosts. Consequently, humans have used aromatic plants for centuries to repel mosquitoes. The repellent effects of plant volatile compounds are mediated through olfactory structures present in the antennae, and maxillary palps of mosquitoes. Mosquito maxillary palps contain capitate-peg sensilla, which house three olfactory sensory neurons, of which two are mainly tuned to either carbon dioxide or octenol - two animal host odorants. However, the third neuron, which expresses the OR49 receptor, has remained without a known ecologically-relevant odorant since its initial discovery. In this study, we used odorant mixtures and terpenoid-rich Cannabis essential oils to investigate the activation of OR49. Our results demonstrate that two monoterpenoids, borneol and camphor, selectively activate OR49, and OR9-expressing neurons, as well as the MD3 glomerulus in the antennal lobe. We confirm that borneol repels female mosquitoes, and knocking out the gene encoding the OR49 receptor suppresses the response of the corresponding olfactory sensory neuron. Importantly, this molecular mechanism of action is conserved across culicine mosquito species, underscoring its significance in their olfactory systems. |
format | Online Article Text |
id | pubmed-10418152 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-104181522023-08-12 The evolution of borneol repellency in culicine mosquitoes Vainer, Yuri Wang, Yinliang Huff, Robert M. Ghaninia, Majid Coutinho-Abreu, Iliano V. Sar-Shalom, Evyatar Ruiz, Carlos Perets, Dor Yakir, Esther Rajamanickam, Dhivya Warburg, Alon Papathanos, Philippos Akbari, Omar S. Ignell, Rickard Riffell, Jeff A. Pitts, R. Jason Bohbot, Jonathan D. bioRxiv Article Insects have developed remarkable adaptations to effectively interact with plant secondary metabolites and utilize them as cues to identify suitable hosts. Consequently, humans have used aromatic plants for centuries to repel mosquitoes. The repellent effects of plant volatile compounds are mediated through olfactory structures present in the antennae, and maxillary palps of mosquitoes. Mosquito maxillary palps contain capitate-peg sensilla, which house three olfactory sensory neurons, of which two are mainly tuned to either carbon dioxide or octenol - two animal host odorants. However, the third neuron, which expresses the OR49 receptor, has remained without a known ecologically-relevant odorant since its initial discovery. In this study, we used odorant mixtures and terpenoid-rich Cannabis essential oils to investigate the activation of OR49. Our results demonstrate that two monoterpenoids, borneol and camphor, selectively activate OR49, and OR9-expressing neurons, as well as the MD3 glomerulus in the antennal lobe. We confirm that borneol repels female mosquitoes, and knocking out the gene encoding the OR49 receptor suppresses the response of the corresponding olfactory sensory neuron. Importantly, this molecular mechanism of action is conserved across culicine mosquito species, underscoring its significance in their olfactory systems. Cold Spring Harbor Laboratory 2023-08-04 /pmc/articles/PMC10418152/ /pubmed/37577635 http://dx.doi.org/10.1101/2023.08.01.548337 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Vainer, Yuri Wang, Yinliang Huff, Robert M. Ghaninia, Majid Coutinho-Abreu, Iliano V. Sar-Shalom, Evyatar Ruiz, Carlos Perets, Dor Yakir, Esther Rajamanickam, Dhivya Warburg, Alon Papathanos, Philippos Akbari, Omar S. Ignell, Rickard Riffell, Jeff A. Pitts, R. Jason Bohbot, Jonathan D. The evolution of borneol repellency in culicine mosquitoes |
title | The evolution of borneol repellency in culicine mosquitoes |
title_full | The evolution of borneol repellency in culicine mosquitoes |
title_fullStr | The evolution of borneol repellency in culicine mosquitoes |
title_full_unstemmed | The evolution of borneol repellency in culicine mosquitoes |
title_short | The evolution of borneol repellency in culicine mosquitoes |
title_sort | evolution of borneol repellency in culicine mosquitoes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10418152/ https://www.ncbi.nlm.nih.gov/pubmed/37577635 http://dx.doi.org/10.1101/2023.08.01.548337 |
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