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Differences in Ratio of Carbon Stable Isotopes among Barley Grain Milling Fractions with Various Concentrations of Beta-Glucans
The grains of three barley varieties were milled and sieved to obtain respective milling fractions with a content of beta-glucans (b-G) from 1.4 to 10.7%. The enriched fraction obtained by the extraction and precipitation contained 24.7% of b-G. The differences between the ratio of stable C carbon i...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420649/ https://www.ncbi.nlm.nih.gov/pubmed/37570708 http://dx.doi.org/10.3390/molecules28155738 |
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author | Levanič, Tom Cigić, Blaž Germ, Mateja Polišenská, Ivana Vaculová, Kateřina Pravst, Igor Kocjan Ačko, Darja Kreft, Ivan |
author_facet | Levanič, Tom Cigić, Blaž Germ, Mateja Polišenská, Ivana Vaculová, Kateřina Pravst, Igor Kocjan Ačko, Darja Kreft, Ivan |
author_sort | Levanič, Tom |
collection | PubMed |
description | The grains of three barley varieties were milled and sieved to obtain respective milling fractions with a content of beta-glucans (b-G) from 1.4 to 10.7%. The enriched fraction obtained by the extraction and precipitation contained 24.7% of b-G. The differences between the ratio of stable C carbon isotopes were established. Milling fractions with coarse particles had more beta-glucans and a more negative ratio of δ(13)C isotope in comparison to the respective intact barley grain. However, the enriched fraction had a less negative isotope ratio. So, it is not expected that the deviation from the stable isotope ratio of grain in milling fractions is the result of the content of b-G, but it depends on other barley grain constituents. In different parts of barley grain, there are substances with different stable isotope ratios, and by milling and sieving, they are assorted to the same milling fraction with most of the b-G. The method for determining the ratio of a stable carbon isotope in diverse barley grain fractions, applied in this investigation, is potentially opening the possibility for an additional method of screening the concentration of bioactive constituents in barley grain. |
format | Online Article Text |
id | pubmed-10420649 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104206492023-08-12 Differences in Ratio of Carbon Stable Isotopes among Barley Grain Milling Fractions with Various Concentrations of Beta-Glucans Levanič, Tom Cigić, Blaž Germ, Mateja Polišenská, Ivana Vaculová, Kateřina Pravst, Igor Kocjan Ačko, Darja Kreft, Ivan Molecules Article The grains of three barley varieties were milled and sieved to obtain respective milling fractions with a content of beta-glucans (b-G) from 1.4 to 10.7%. The enriched fraction obtained by the extraction and precipitation contained 24.7% of b-G. The differences between the ratio of stable C carbon isotopes were established. Milling fractions with coarse particles had more beta-glucans and a more negative ratio of δ(13)C isotope in comparison to the respective intact barley grain. However, the enriched fraction had a less negative isotope ratio. So, it is not expected that the deviation from the stable isotope ratio of grain in milling fractions is the result of the content of b-G, but it depends on other barley grain constituents. In different parts of barley grain, there are substances with different stable isotope ratios, and by milling and sieving, they are assorted to the same milling fraction with most of the b-G. The method for determining the ratio of a stable carbon isotope in diverse barley grain fractions, applied in this investigation, is potentially opening the possibility for an additional method of screening the concentration of bioactive constituents in barley grain. MDPI 2023-07-29 /pmc/articles/PMC10420649/ /pubmed/37570708 http://dx.doi.org/10.3390/molecules28155738 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Levanič, Tom Cigić, Blaž Germ, Mateja Polišenská, Ivana Vaculová, Kateřina Pravst, Igor Kocjan Ačko, Darja Kreft, Ivan Differences in Ratio of Carbon Stable Isotopes among Barley Grain Milling Fractions with Various Concentrations of Beta-Glucans |
title | Differences in Ratio of Carbon Stable Isotopes among Barley Grain Milling Fractions with Various Concentrations of Beta-Glucans |
title_full | Differences in Ratio of Carbon Stable Isotopes among Barley Grain Milling Fractions with Various Concentrations of Beta-Glucans |
title_fullStr | Differences in Ratio of Carbon Stable Isotopes among Barley Grain Milling Fractions with Various Concentrations of Beta-Glucans |
title_full_unstemmed | Differences in Ratio of Carbon Stable Isotopes among Barley Grain Milling Fractions with Various Concentrations of Beta-Glucans |
title_short | Differences in Ratio of Carbon Stable Isotopes among Barley Grain Milling Fractions with Various Concentrations of Beta-Glucans |
title_sort | differences in ratio of carbon stable isotopes among barley grain milling fractions with various concentrations of beta-glucans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10420649/ https://www.ncbi.nlm.nih.gov/pubmed/37570708 http://dx.doi.org/10.3390/molecules28155738 |
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