Solid-phase capture and profiling of open chromatin by spatial ATAC

Current methods for epigenomic profiling are limited in their ability to obtain genome-wide information with spatial resolution. We introduce spatial ATAC, a method that integrates transposase-accessible chromatin profiling in tissue sections with barcoded solid-phase capture to perform spatially re...

Descripción completa

Detalles Bibliográficos
Autores principales: Llorens-Bobadilla, Enric, Zamboni, Margherita, Marklund, Maja, Bhalla, Nayanika, Chen, Xinsong, Hartman, Johan, Frisén, Jonas, Ståhl, Patrik L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2023
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10421738/
https://www.ncbi.nlm.nih.gov/pubmed/36604544
http://dx.doi.org/10.1038/s41587-022-01603-9
Descripción
Sumario:Current methods for epigenomic profiling are limited in their ability to obtain genome-wide information with spatial resolution. We introduce spatial ATAC, a method that integrates transposase-accessible chromatin profiling in tissue sections with barcoded solid-phase capture to perform spatially resolved epigenomics. We show that spatial ATAC enables the discovery of the regulatory programs underlying spatial gene expression during mouse organogenesis, lineage differentiation and in human pathology.

Ejemplares similares