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exo-FISH: Protocol for detecting DNA breaks in repetitive regions of mammalian genomes

Detecting DNA breaks in defined regions of the genome is critical to advancing our understanding of genome stability maintenance. Here, we present exo-FISH, a protocol to label exposed single-stranded DNA in defined repetitive regions of mammalian genomes by combining in vitro restriction enzyme dig...

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Detalles Bibliográficos
Autores principales: Saayman, Xanita, Graham, Emily, Leung, Chin Wei Brian, Esashi, Fumiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10425934/
https://www.ncbi.nlm.nih.gov/pubmed/37549036
http://dx.doi.org/10.1016/j.xpro.2023.102487