Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum

Here, we present a protocol for the design, fabrication, and usage of a polydimethylsiloxane (PDMS)-based chip for dynamic microfluidic single-cell cultivation of Corynebacterium glutamicum. We describe steps for flow profile establishment and biological preparation. We then detail time-lapse imagin...

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Detalles Bibliográficos
Autores principales: Blöbaum, Luisa, Täuber, Sarah, Grünberger, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10425941/
https://www.ncbi.nlm.nih.gov/pubmed/37543944
http://dx.doi.org/10.1016/j.xpro.2023.102436

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10425941/
https://www.ncbi.nlm.nih.gov/pubmed/37543944
http://dx.doi.org/10.1016/j.xpro.2023.102436

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