Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum

Here, we present a protocol for the design, fabrication, and usage of a polydimethylsiloxane (PDMS)-based chip for dynamic microfluidic single-cell cultivation of Corynebacterium glutamicum. We describe steps for flow profile establishment and biological preparation. We then detail time-lapse imagin...

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Detalles Bibliográficos
Autores principales: Blöbaum, Luisa, Täuber, Sarah, Grünberger, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10425941/
https://www.ncbi.nlm.nih.gov/pubmed/37543944
http://dx.doi.org/10.1016/j.xpro.2023.102436
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author Blöbaum, Luisa
Täuber, Sarah
Grünberger, Alexander
author_facet Blöbaum, Luisa
Täuber, Sarah
Grünberger, Alexander
author_sort Blöbaum, Luisa
collection PubMed
description Here, we present a protocol for the design, fabrication, and usage of a polydimethylsiloxane (PDMS)-based chip for dynamic microfluidic single-cell cultivation of Corynebacterium glutamicum. We describe steps for flow profile establishment and biological preparation. We then detail time-lapse imaging to observe reactions of C. glutamicum to repeated environmental changes in the range of seconds. This system can be adapted to other organisms with a cell wall and soluble non-gaseous environmental factors like nutrients. For complete details on the use and execution of this protocol, please refer to Täuber et al..(1)
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spelling pubmed-104259412023-08-16 Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum Blöbaum, Luisa Täuber, Sarah Grünberger, Alexander STAR Protoc Protocol Here, we present a protocol for the design, fabrication, and usage of a polydimethylsiloxane (PDMS)-based chip for dynamic microfluidic single-cell cultivation of Corynebacterium glutamicum. We describe steps for flow profile establishment and biological preparation. We then detail time-lapse imaging to observe reactions of C. glutamicum to repeated environmental changes in the range of seconds. This system can be adapted to other organisms with a cell wall and soluble non-gaseous environmental factors like nutrients. For complete details on the use and execution of this protocol, please refer to Täuber et al..(1) Elsevier 2023-08-06 /pmc/articles/PMC10425941/ /pubmed/37543944 http://dx.doi.org/10.1016/j.xpro.2023.102436 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Blöbaum, Luisa
Täuber, Sarah
Grünberger, Alexander
Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum
title Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum
title_full Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum
title_fullStr Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum
title_full_unstemmed Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum
title_short Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum
title_sort protocol to perform dynamic microfluidic single-cell cultivation of c. glutamicum
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10425941/
https://www.ncbi.nlm.nih.gov/pubmed/37543944
http://dx.doi.org/10.1016/j.xpro.2023.102436
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