Cargando…

Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum

Here, we present a protocol for the design, fabrication, and usage of a polydimethylsiloxane (PDMS)-based chip for dynamic microfluidic single-cell cultivation of Corynebacterium glutamicum. We describe steps for flow profile establishment and biological preparation. We then detail time-lapse imagin...

Descripción completa

Detalles Bibliográficos
Autores principales:	Blöbaum, Luisa, Täuber, Sarah, Grünberger, Alexander
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10425941/ https://www.ncbi.nlm.nih.gov/pubmed/37543944 http://dx.doi.org/10.1016/j.xpro.2023.102436

Ejemplares similares

How to Perform a Microfluidic Cultivation Experiment—A Guideline to Success
por: Täuber, Sarah, et al.
Publicado: (2021)

Growth Response and Recovery of Corynebacterium glutamicum Colonies on Single-Cell Level Upon Defined pH Stress Pulses
por: Täuber, Sarah, et al.
Publicado: (2021)

Reliable cell retention of mammalian suspension cells in microfluidic cultivation chambers
por: Schmitz, Julian, et al.
Publicado: (2023)

A microfluidic photobioreactor for simultaneous observation and cultivation of single microalgal cells or cell aggregates
por: Westerwalbesloh, Christoph, et al.
Publicado: (2019)

Comparative Single-Cell Analysis of Different E. coli Expression Systems during Microfluidic Cultivation
por: Binder, Dennis, et al.
Publicado: (2016)

Production of Food and Feed Additives From Non-food-competing Feedstocks: Valorizing N-acetylmuramic Acid for Amino Acid and Carotenoid Fermentation With Corynebacterium glutamicum
por: Sgobba, Elvira, et al.
Publicado: (2018)

Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli
por: Bruggeman, Christine W., et al.
Publicado: (2023)

Human Blood Serum Induces p38-MAPK- and Hsp27-Dependent Migration Dynamics of Adult Human Cardiac Stem Cells: Single-Cell Analysis via a Microfluidic-Based Cultivation Platform
por: Höving, Anna L., et al.
Publicado: (2021)

Protocol of living cell separation using the microfluidic dielectrophoresis integrated chip
por: Koba, Kazumi, et al.
Publicado: (2022)

Effect of fed-batch and chemostat cultivation processes of C. glutamicum CP for L-leucine production
por: Zhang, Yufu, et al.
Publicado: (2021)

Microfluidics for Biotechnology: Bridging Gaps to Foster Microfluidic Applications
por: Ortseifen, Vera, et al.
Publicado: (2020)

Protocol for bioelectrochemical enrichment, cultivation, and characterization of extreme electroactive microorganisms
por: Singh, Ramandeep, et al.
Publicado: (2022)

A live-imaging protocol for tracking receptor dynamics in single cells
por: Huang, Yibin, et al.
Publicado: (2022)

Process inhomogeneity leads to rapid side product turnover in cultivation of Corynebacterium glutamicum
por: Käß, Friedrich, et al.
Publicado: (2014)

Growth and eGFP Production of CHO-K1 Suspension Cells Cultivated From Single Cell to Laboratory Scale
por: Schmitz, Julian, et al.
Publicado: (2021)

Isolation, cultivation and immunostaining of single myofibers: An improved approach to study the behavior of satellite cells
por: Lim, Chai Ling, et al.
Publicado: (2018)

Mu-driven transposition of recombinant mini-Mu unit DNA in the Corynebacterium glutamicum chromosome
por: Gorshkova, Natalya V., et al.
Publicado: (2018)

A Micropillar Array Based Microfluidic Device for Rare Cell Detection and Single-Cell Proteomics
por: Chen, Kangfu, et al.
Publicado: (2023)

Microfluidics for single-cell lineage tracking over time to characterize transmission of phenotypes in Saccharomyces cerevisiae
por: Bheda, Poonam, et al.
Publicado: (2020)

Dissociation and inDrops microfluidic encapsulation of human gut tissues for single-cell atlasing studies
por: Simmons, Alan J., et al.
Publicado: (2022)

Automated Chemotactic Sorting and Single-cell Cultivation of Microbes using Droplet Microfluidics
por: Dong, Libing, et al.
Publicado: (2016)

Genomics Characterization of an Engineered Corynebacterium glutamicum in Bioreactor Cultivation Under Ionic Liquid Stress
por: Banerjee, Deepanwita, et al.
Publicado: (2021)

A Protocol for Fabrication and on-Chip Cell Culture to Recreate PAH-Afflicted Pulmonary Artery on a Microfluidic Device
por: Sarkar, Tanoy, et al.
Publicado: (2022)

Application of a Genetically Encoded Biosensor for Live Cell Imaging of L-Valine Production in Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum Strains
por: Mustafi, Nurije, et al.
Publicado: (2014)

Protocol to determine antibody affinity and concentration in complex solutions using microfluidic antibody affinity profiling
por: Emmenegger, Marc, et al.
Publicado: (2023)

Isolation and in vitro cultivation of adrenal cells from mice
por: Friedrich, Linda, et al.
Publicado: (2021)

Isolation and Cultivation of Adipose-Derived Mesenchymal Stem Cells Originating from the Infrapatellar Fat Pad Differentiated with Blood Products: Method and Protocol
por: Neubauer, Markus, et al.
Publicado: (2022)

Two Methods for the Isolation and Cultivation of Porcine Primary Corneal Cells
por: Netto, Alice Rocha Teixeira, et al.
Publicado: (2023)

The Industrial Organism Corynebacterium glutamicum Requires Mycothiol as Antioxidant to Resist Against Oxidative Stress in Bioreactor Cultivations
por: Hartmann, Fabian Stefan Franz, et al.
Publicado: (2020)

Droplet-Based Microfluidic High Throughput Screening of Corynebacterium glutamicum for Efficient Heterologous Protein Production and Secretion
por: Balasubramanian, Suvasini, et al.
Publicado: (2021)

Protocol to develop a microfluidic human corneal barrier-on-a-chip to evaluate the corneal epithelial wound repair process
por: Yu, Zitong, et al.
Publicado: (2023)

Protocol to study the oligomeric organization of single-span transmembrane peptides using molecular dynamics simulations
por: Sica, Mauricio P., et al.
Publicado: (2022)

In Vitro Cultivation of the Syphilis Spirochete Treponema pallidum
por: Edmondson, Diane G., et al.
Publicado: (2021)

Online estimation of changing metabolic capacities in continuous Corynebacterium glutamicum cultivations growing on a complex sugar mixture
por: Sinner, Peter, et al.
Publicado: (2021)

Protocol for single-molecule fluorescence recovery after photobleaching microscopy to analyze the dynamics and spatial locations of nuclear transmembrane proteins in live cells
por: Tingey, Mark, et al.
Publicado: (2021)

Microfluidic encapsulation of Xenopus laevis cell-free extracts using hydrogel photolithography
por: Geisterfer, Zachary M., et al.
Publicado: (2020)

Protocol for electrical modulation of ERK signaling dynamics in cell lines
por: Zhu, Kan, et al.
Publicado: (2022)

Monitoring of population dynamics of Corynebacterium glutamicum by multiparameter flow cytometry
por: Neumeyer, Andrea, et al.
Publicado: (2013)

Protocol for Dissection and Dissociation of Zebrafish Telencephalon for Single-Cell Sequencing
por: Cosacak, Mehmet Ilyas, et al.
Publicado: (2020)

Protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using Seahorse XF96
por: Miguel, Verónica, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_52r2ri0p8ssafhulk8mda5src8