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Modeling suggests that virion production cycles within individual cells is key to understanding acute hepatitis B virus infection kinetics
Hepatitis B virus (HBV) infection kinetics in immunodeficient mice reconstituted with humanized livers from inoculation to steady state is highly dynamic despite the absence of an adaptive immune response. To recapitulate the multiphasic viral kinetic patterns, we developed an agent-based model that...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10426918/ https://www.ncbi.nlm.nih.gov/pubmed/37535676 http://dx.doi.org/10.1371/journal.pcbi.1011309 |
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author | Hailegiorgis, Atesmachew Ishida, Yuji Collier, Nicholson Imamura, Michio Shi, Zhenzhen Reinharz, Vladimir Tsuge, Masataka Barash, Danny Hiraga, Nobuhiko Yokomichi, Hiroshi Tateno, Chise Ozik, Jonathan Uprichard, Susan L. Chayama, Kazuaki Dahari, Harel |
author_facet | Hailegiorgis, Atesmachew Ishida, Yuji Collier, Nicholson Imamura, Michio Shi, Zhenzhen Reinharz, Vladimir Tsuge, Masataka Barash, Danny Hiraga, Nobuhiko Yokomichi, Hiroshi Tateno, Chise Ozik, Jonathan Uprichard, Susan L. Chayama, Kazuaki Dahari, Harel |
author_sort | Hailegiorgis, Atesmachew |
collection | PubMed |
description | Hepatitis B virus (HBV) infection kinetics in immunodeficient mice reconstituted with humanized livers from inoculation to steady state is highly dynamic despite the absence of an adaptive immune response. To recapitulate the multiphasic viral kinetic patterns, we developed an agent-based model that includes intracellular virion production cycles reflecting the cyclic nature of each individual virus lifecycle. The model fits the data well predicting an increase in production cycles initially starting with a long production cycle of 1 virion per 20 hours that gradually reaches 1 virion per hour after approximately 3–4 days before virion production increases dramatically to reach to a steady state rate of 4 virions per hour per cell. Together, modeling suggests that it is the cyclic nature of the virus lifecycle combined with an initial slow but increasing rate of HBV production from each cell that plays a role in generating the observed multiphasic HBV kinetic patterns in humanized mice. |
format | Online Article Text |
id | pubmed-10426918 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-104269182023-08-16 Modeling suggests that virion production cycles within individual cells is key to understanding acute hepatitis B virus infection kinetics Hailegiorgis, Atesmachew Ishida, Yuji Collier, Nicholson Imamura, Michio Shi, Zhenzhen Reinharz, Vladimir Tsuge, Masataka Barash, Danny Hiraga, Nobuhiko Yokomichi, Hiroshi Tateno, Chise Ozik, Jonathan Uprichard, Susan L. Chayama, Kazuaki Dahari, Harel PLoS Comput Biol Research Article Hepatitis B virus (HBV) infection kinetics in immunodeficient mice reconstituted with humanized livers from inoculation to steady state is highly dynamic despite the absence of an adaptive immune response. To recapitulate the multiphasic viral kinetic patterns, we developed an agent-based model that includes intracellular virion production cycles reflecting the cyclic nature of each individual virus lifecycle. The model fits the data well predicting an increase in production cycles initially starting with a long production cycle of 1 virion per 20 hours that gradually reaches 1 virion per hour after approximately 3–4 days before virion production increases dramatically to reach to a steady state rate of 4 virions per hour per cell. Together, modeling suggests that it is the cyclic nature of the virus lifecycle combined with an initial slow but increasing rate of HBV production from each cell that plays a role in generating the observed multiphasic HBV kinetic patterns in humanized mice. Public Library of Science 2023-08-03 /pmc/articles/PMC10426918/ /pubmed/37535676 http://dx.doi.org/10.1371/journal.pcbi.1011309 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Hailegiorgis, Atesmachew Ishida, Yuji Collier, Nicholson Imamura, Michio Shi, Zhenzhen Reinharz, Vladimir Tsuge, Masataka Barash, Danny Hiraga, Nobuhiko Yokomichi, Hiroshi Tateno, Chise Ozik, Jonathan Uprichard, Susan L. Chayama, Kazuaki Dahari, Harel Modeling suggests that virion production cycles within individual cells is key to understanding acute hepatitis B virus infection kinetics |
title | Modeling suggests that virion production cycles within individual cells is key to understanding acute hepatitis B virus infection kinetics |
title_full | Modeling suggests that virion production cycles within individual cells is key to understanding acute hepatitis B virus infection kinetics |
title_fullStr | Modeling suggests that virion production cycles within individual cells is key to understanding acute hepatitis B virus infection kinetics |
title_full_unstemmed | Modeling suggests that virion production cycles within individual cells is key to understanding acute hepatitis B virus infection kinetics |
title_short | Modeling suggests that virion production cycles within individual cells is key to understanding acute hepatitis B virus infection kinetics |
title_sort | modeling suggests that virion production cycles within individual cells is key to understanding acute hepatitis b virus infection kinetics |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10426918/ https://www.ncbi.nlm.nih.gov/pubmed/37535676 http://dx.doi.org/10.1371/journal.pcbi.1011309 |
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