Efficient delivery of a large-size Cas9-EGFP vector in porcine fetal fibroblasts using a Lonza 4D-Nucleofector system

BACKGROUND: Porcine fetal fibroblasts (PFFs) are important donor cells for generating genetically modified pigs, but the transfection efficiencies of PFFs are often unsatisfactory especially when large-size vectors are to be delivered. In this study, we aimed to optimize the transfection conditions...

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Detalles Bibliográficos
Autores principales: Liu, Weiwei, Wang, Xiaoguo, Liu, Ruirong, Liao, Yaya, Peng, Zhiwei, Jiang, Haoyun, Jing, Qiqi, Xing, Yuyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10428654/
https://www.ncbi.nlm.nih.gov/pubmed/37587435
http://dx.doi.org/10.1186/s12896-023-00799-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10428654/
https://www.ncbi.nlm.nih.gov/pubmed/37587435
http://dx.doi.org/10.1186/s12896-023-00799-1

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