Protocol to establish an oviduct epithelial cell line derived from Gallus gallus using Percoll for in vitro validation of recombinant proteins

In vitro validation of therapeutic and recombinant proteins expressed from transgenic chickens is limited by the co-culture of fibroblasts. Here, we present a protocol for isolating pure epithelial cells derived from the magnum tubular glands of the chicken oviduct. We describe steps for preparing s...

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Detalles Bibliográficos
Autores principales: Loubser, Chiron, Nikitina, Natalya V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10432238/
https://www.ncbi.nlm.nih.gov/pubmed/37542716
http://dx.doi.org/10.1016/j.xpro.2023.102495

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10432238/
https://www.ncbi.nlm.nih.gov/pubmed/37542716
http://dx.doi.org/10.1016/j.xpro.2023.102495

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