Markerless bacterial artificial chromosome manipulation method by red proteins of phage λ mediated homologous recombination utilizing fluorescent proteins for both positive and counter selection

Manipulating viral genomes is an essential technique in reverse genetics and recombinant vaccine development. A strategy for manipulating large viral genomes involves introducing their entire genome into bacterial artificial chromosomes and employing Escherichia coli genetic tools. For sequence mani...

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Detalles Bibliográficos
Autores principales: Yoshikawa, Tomoki, Misu, Masayasu, Kurosu, Takeshi, Takamatsu, Yuki, Sugimoto, Satoko, Shimojima, Masayuki, Ebihara, Hideki, Saijo, Masayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10432722/
https://www.ncbi.nlm.nih.gov/pubmed/37600421
http://dx.doi.org/10.1016/j.heliyon.2023.e18983

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10432722/
https://www.ncbi.nlm.nih.gov/pubmed/37600421
http://dx.doi.org/10.1016/j.heliyon.2023.e18983

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