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Optimisation of recombinant TNFα production in Escherichia coli using GFP fusions and flow cytometry
Escherichia coli is commonly used industrially to manufacture recombinant proteins for biopharmaceutical applications, as well as in academic and industrial settings for R&D purposes. Optimisation of recombinant protein production remains problematic as many proteins are difficult to make, and p...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10433901/ https://www.ncbi.nlm.nih.gov/pubmed/37600304 http://dx.doi.org/10.3389/fbioe.2023.1171823 |