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Development and Application of a Nanobody-Based Competitive ELISA for Detecting Antibodies against Hepatitis E Virus from Humans and Domestic Animals

Hepatitis E virus (HEV) is a zoonotic pathogen that is widespread worldwide. At present, most enzyme-linked immunosorbent assay (ELISA) kits only detect antibodies against human HEV. In this study, a nanobody-horseradish peroxidase (HRP) fusion protein-based competitive ELISA (cELISA) with more conv...

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Detalles Bibliográficos
Autores principales: Chen, Yiyang, Zhang, Meimei, Chen, Tianxiang, Wang, Jiaxi, Zhao, Qin, Zhou, En-Min, Liu, Baoyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434039/
https://www.ncbi.nlm.nih.gov/pubmed/37347160
http://dx.doi.org/10.1128/spectrum.03607-22