Protocol for human brain organoid transplantation into a rat visual cortex to model neural repair

Human stem-cell-derived organoids represent a promising substrate for transplantation-based neural repair. Here, we describe a protocol for transplanting forebrain organoids into an injured adult rat visual cortex. This protocol includes surgical details for craniectomy, aspiration injury, organoid...

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Detalles Bibliográficos
Autores principales: Jgamadze, Dennis, Harary, Paul M., Castellanos, Mackenzie, Blue, Rachel, Song, Hongjun, Ming, Guo-li, Chen, H. Isaac
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10436235/
https://www.ncbi.nlm.nih.gov/pubmed/37585295
http://dx.doi.org/10.1016/j.xpro.2023.102470
Descripción
Sumario:Human stem-cell-derived organoids represent a promising substrate for transplantation-based neural repair. Here, we describe a protocol for transplanting forebrain organoids into an injured adult rat visual cortex. This protocol includes surgical details for craniectomy, aspiration injury, organoid transplantation, and cranioplasty. This platform represents a valuable tool for investigating the efficacy of organoids as structured grafts for neural repair. For complete details on the use and execution of this protocol, please refer to Jgamadze et al.(1)

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