Protocol for human brain organoid transplantation into a rat visual cortex to model neural repair

Human stem-cell-derived organoids represent a promising substrate for transplantation-based neural repair. Here, we describe a protocol for transplanting forebrain organoids into an injured adult rat visual cortex. This protocol includes surgical details for craniectomy, aspiration injury, organoid...

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Detalles Bibliográficos
Autores principales: Jgamadze, Dennis, Harary, Paul M., Castellanos, Mackenzie, Blue, Rachel, Song, Hongjun, Ming, Guo-li, Chen, H. Isaac
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10436235/
https://www.ncbi.nlm.nih.gov/pubmed/37585295
http://dx.doi.org/10.1016/j.xpro.2023.102470
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author Jgamadze, Dennis
Harary, Paul M.
Castellanos, Mackenzie
Blue, Rachel
Song, Hongjun
Ming, Guo-li
Chen, H. Isaac
author_facet Jgamadze, Dennis
Harary, Paul M.
Castellanos, Mackenzie
Blue, Rachel
Song, Hongjun
Ming, Guo-li
Chen, H. Isaac
author_sort Jgamadze, Dennis
collection PubMed
description Human stem-cell-derived organoids represent a promising substrate for transplantation-based neural repair. Here, we describe a protocol for transplanting forebrain organoids into an injured adult rat visual cortex. This protocol includes surgical details for craniectomy, aspiration injury, organoid transplantation, and cranioplasty. This platform represents a valuable tool for investigating the efficacy of organoids as structured grafts for neural repair. For complete details on the use and execution of this protocol, please refer to Jgamadze et al.(1)
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spelling pubmed-104362352023-08-19 Protocol for human brain organoid transplantation into a rat visual cortex to model neural repair Jgamadze, Dennis Harary, Paul M. Castellanos, Mackenzie Blue, Rachel Song, Hongjun Ming, Guo-li Chen, H. Isaac STAR Protoc Protocol Human stem-cell-derived organoids represent a promising substrate for transplantation-based neural repair. Here, we describe a protocol for transplanting forebrain organoids into an injured adult rat visual cortex. This protocol includes surgical details for craniectomy, aspiration injury, organoid transplantation, and cranioplasty. This platform represents a valuable tool for investigating the efficacy of organoids as structured grafts for neural repair. For complete details on the use and execution of this protocol, please refer to Jgamadze et al.(1) Elsevier 2023-08-15 /pmc/articles/PMC10436235/ /pubmed/37585295 http://dx.doi.org/10.1016/j.xpro.2023.102470 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Jgamadze, Dennis
Harary, Paul M.
Castellanos, Mackenzie
Blue, Rachel
Song, Hongjun
Ming, Guo-li
Chen, H. Isaac
Protocol for human brain organoid transplantation into a rat visual cortex to model neural repair
title Protocol for human brain organoid transplantation into a rat visual cortex to model neural repair
title_full Protocol for human brain organoid transplantation into a rat visual cortex to model neural repair
title_fullStr Protocol for human brain organoid transplantation into a rat visual cortex to model neural repair
title_full_unstemmed Protocol for human brain organoid transplantation into a rat visual cortex to model neural repair
title_short Protocol for human brain organoid transplantation into a rat visual cortex to model neural repair
title_sort protocol for human brain organoid transplantation into a rat visual cortex to model neural repair
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10436235/
https://www.ncbi.nlm.nih.gov/pubmed/37585295
http://dx.doi.org/10.1016/j.xpro.2023.102470
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