A DNA assembly toolkit to unlock the CRISPR/Cas9 potential for metabolic engineering

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CRISPR/Cas9-based technologies are revolutionising the way we engineer microbial cells. One of the key advantages of CRISPR in strain design is that it enables chromosomal integration of marker-free DNA, eliminating laborious and often inefficient marker recovery procedures. Despite the benefits, as...

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Detalles Bibliográficos
Autores principales: Yuzbashev, Tigran V., Yuzbasheva, Evgeniya Y., Melkina, Olga E., Patel, Davina,  Bubnov, Dmitrii, Dietz, Heiko, Ledesma-Amaro, Rodrigo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439232/
https://www.ncbi.nlm.nih.gov/pubmed/37596335
http://dx.doi.org/10.1038/s42003-023-05202-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10439232/
https://www.ncbi.nlm.nih.gov/pubmed/37596335
http://dx.doi.org/10.1038/s42003-023-05202-5

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