Protocol for measuring BRAF autoinhibition in live cells using a proximity-based NanoBRET assay

BRAF is frequently activated via mutation in human cancer and the RASopathy syndromes; however, for BRAF activation to occur, autoinhibitory interactions between the regulatory and catalytic domains must be relieved. Here, we present a proximity-based NanoBRET (bioluminescence resonance energy trans...

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Detalles Bibliográficos
Autores principales: Spencer-Smith, Russell, Morrison, Deborah K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10440347/
https://www.ncbi.nlm.nih.gov/pubmed/37590148
http://dx.doi.org/10.1016/j.xpro.2023.102461

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10440347/
https://www.ncbi.nlm.nih.gov/pubmed/37590148
http://dx.doi.org/10.1016/j.xpro.2023.102461

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