Characterizing CD4 T cell differentiation in mouse small intestine using T cell transfer, lamina propria preparation, and flow cytometry

Studying gene function in T cells is crucial for understanding physiology and disease pathogenesis. Here, we provide a protocol to examine the role of specific genes in CD4(+) T cell differentiation in the intestine. We describe steps for isolating naïve CD4(+) T cells from mouse spleens and transfe...

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Detalles Bibliográficos
Autores principales: Duan, Jinzhi, Sun, Yanan, Matute, Juan D., Blumberg, Richard S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10440592/
https://www.ncbi.nlm.nih.gov/pubmed/37566548
http://dx.doi.org/10.1016/j.xpro.2023.102485

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10440592/
https://www.ncbi.nlm.nih.gov/pubmed/37566548
http://dx.doi.org/10.1016/j.xpro.2023.102485

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