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Characterizing CD4 T cell differentiation in mouse small intestine using T cell transfer, lamina propria preparation, and flow cytometry
Studying gene function in T cells is crucial for understanding physiology and disease pathogenesis. Here, we provide a protocol to examine the role of specific genes in CD4(+) T cell differentiation in the intestine. We describe steps for isolating naïve CD4(+) T cells from mouse spleens and transfe...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10440592/ https://www.ncbi.nlm.nih.gov/pubmed/37566548 http://dx.doi.org/10.1016/j.xpro.2023.102485 |