Targeted In Vivo Mutagenesis in Yeast Using CRISPR/Cas9 and Hyperactive Cytidine and Adenine Deaminases

[Image: see text] Directed evolution is a preferred strategy to improve the function of proteins such as enzymes that act as bottlenecks in metabolic pathways. Common directed evolution approaches rely on error-prone PCR-based libraries where the number of possible variants is usually limited by cel...

Descripción completa

Detalles Bibliográficos
Autores principales: Skrekas, Christos, Limeta, Angelo, Siewers, Verena, David, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443040/
https://www.ncbi.nlm.nih.gov/pubmed/37486333
http://dx.doi.org/10.1021/acssynbio.2c00690

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443040/
https://www.ncbi.nlm.nih.gov/pubmed/37486333
http://dx.doi.org/10.1021/acssynbio.2c00690

Ejemplares similares