Cost-Effective Protein Production in CHO Cells Following Polyethylenimine-Mediated Gene Delivery Showcased by the Production and Crystallization of Antibody Fabs

Laboratory production of recombinant mammalian proteins, particularly antibodies, requires an expression pipeline assuring sufficient yield and correct folding with appropriate posttranslational modifications. Transient gene expression (TGE) in the suspension-adapted Chinese Hamster Ovary (CHO) cell...

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Detalles Bibliográficos
Autores principales: Meskova, Klaudia, Martonova, Katarina, Hrasnova, Patricia, Sinska, Kristina, Skrabanova, Michaela, Fialova, Lubica, Njemoga, Stefana, Cehlar, Ondrej, Parmar, Olga, Kolenko, Petr, Pevala, Vladimir, Skrabana, Rostislav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443350/
https://www.ncbi.nlm.nih.gov/pubmed/37606435
http://dx.doi.org/10.3390/antib12030051

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10443350/
https://www.ncbi.nlm.nih.gov/pubmed/37606435
http://dx.doi.org/10.3390/antib12030051

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