Amino acid analysis for peptide quantitation using reversed-phase liquid chromatography combined with multiple reaction monitoring mass spectrometry

Amino acid analysis (AAA) can be used for absolute quantitation of standard peptides after acid hydrolysis using 6 M HCl. Obtained individual amino acids can then be quantified by liquid chromatography-mass spectrometry (LC–MS). Achieving baseline separation of non-derivatized amino acids is challen...

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Detalles Bibliográficos
Autores principales: Qasrawi, Deema O., Petrotchenko, Evgeniy V., Borchers, Christoph H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10444640/
https://www.ncbi.nlm.nih.gov/pubmed/37468754
http://dx.doi.org/10.1007/s00216-023-04840-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10444640/
https://www.ncbi.nlm.nih.gov/pubmed/37468754
http://dx.doi.org/10.1007/s00216-023-04840-2

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