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Efficient splicing of the CPE intein derived from directed evolution of the Cryptococcus neoformans PRP8 intein : Directed evolution of the Cryptococcus neoformans PRP8 intein

Intein-mediated protein splicing has been widely used in protein engineering; however, the splicing efficiency and extein specificity usually limit its further application. Thus, there is a demand for more general inteins that can overcome these limitations. Here, we study the trans-splicing of CPE...

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Detalles Bibliográficos
Autores principales: Zhan, Qin, Shi, Changhua, Jiang, Yu, Gao, Xianling, Lin, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448054/
https://www.ncbi.nlm.nih.gov/pubmed/37489009
http://dx.doi.org/10.3724/abbs.2023135