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Efficient splicing of the CPE intein derived from directed evolution of the Cryptococcus neoformans PRP8 intein : Directed evolution of the Cryptococcus neoformans PRP8 intein
Intein-mediated protein splicing has been widely used in protein engineering; however, the splicing efficiency and extein specificity usually limit its further application. Thus, there is a demand for more general inteins that can overcome these limitations. Here, we study the trans-splicing of CPE...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448054/ https://www.ncbi.nlm.nih.gov/pubmed/37489009 http://dx.doi.org/10.3724/abbs.2023135 |