Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter

Upon exposure to a bacterial pore-forming toxin, enterocytes rapidly purge their apical cytoplasm into the gut lumen, resulting in a thin intestinal epithelium. The enterocytes regain their original shape and thickness within 16 h after the ingestion of the bacteria. Here, we show that the regrowth...

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Autores principales: Socha, Catherine, Pais, Inês S., Lee, Kwang-Zin, Liu, Jiyong, Liégeois, Samuel, Lestradet, Matthieu, Ferrandon, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448536/
https://www.ncbi.nlm.nih.gov/pubmed/37636057
http://dx.doi.org/10.1016/j.isci.2023.107490
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author Socha, Catherine
Pais, Inês S.
Lee, Kwang-Zin
Liu, Jiyong
Liégeois, Samuel
Lestradet, Matthieu
Ferrandon, Dominique
author_facet Socha, Catherine
Pais, Inês S.
Lee, Kwang-Zin
Liu, Jiyong
Liégeois, Samuel
Lestradet, Matthieu
Ferrandon, Dominique
author_sort Socha, Catherine
collection PubMed
description Upon exposure to a bacterial pore-forming toxin, enterocytes rapidly purge their apical cytoplasm into the gut lumen, resulting in a thin intestinal epithelium. The enterocytes regain their original shape and thickness within 16 h after the ingestion of the bacteria. Here, we show that the regrowth of Drosophila enterocytes entails an inversion of metabolic fluxes from the organism back toward the intestine. We identify a proton-assisted transporter, Arcus, that is required for the reverse absorption of amino acids and the timely recovery of the intestinal epithelium. Arcus is required for a peak of amino acids appearing in the hemolymph shortly after infection. The regrowth of enterocytes involves the insulin signaling pathway and Myc. The purge decreases Myc mRNA levels, which subsequently remain at low levels in the arcus mutant. Interestingly, the action of arcus and Myc in the intestinal epithelium is not cell-autonomous, suggesting amino acid fluxes within the intestinal epithelium.
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spelling pubmed-104485362023-08-25 Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter Socha, Catherine Pais, Inês S. Lee, Kwang-Zin Liu, Jiyong Liégeois, Samuel Lestradet, Matthieu Ferrandon, Dominique iScience Article Upon exposure to a bacterial pore-forming toxin, enterocytes rapidly purge their apical cytoplasm into the gut lumen, resulting in a thin intestinal epithelium. The enterocytes regain their original shape and thickness within 16 h after the ingestion of the bacteria. Here, we show that the regrowth of Drosophila enterocytes entails an inversion of metabolic fluxes from the organism back toward the intestine. We identify a proton-assisted transporter, Arcus, that is required for the reverse absorption of amino acids and the timely recovery of the intestinal epithelium. Arcus is required for a peak of amino acids appearing in the hemolymph shortly after infection. The regrowth of enterocytes involves the insulin signaling pathway and Myc. The purge decreases Myc mRNA levels, which subsequently remain at low levels in the arcus mutant. Interestingly, the action of arcus and Myc in the intestinal epithelium is not cell-autonomous, suggesting amino acid fluxes within the intestinal epithelium. Elsevier 2023-07-27 /pmc/articles/PMC10448536/ /pubmed/37636057 http://dx.doi.org/10.1016/j.isci.2023.107490 Text en © 2023. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Socha, Catherine
Pais, Inês S.
Lee, Kwang-Zin
Liu, Jiyong
Liégeois, Samuel
Lestradet, Matthieu
Ferrandon, Dominique
Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter
title Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter
title_full Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter
title_fullStr Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter
title_full_unstemmed Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter
title_short Fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter
title_sort fast drosophila enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448536/
https://www.ncbi.nlm.nih.gov/pubmed/37636057
http://dx.doi.org/10.1016/j.isci.2023.107490
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