All-synchronized picosecond pulses and time-gated detection improve the spatial resolution of two-photon STED microscopy in brain tissue imaging

Super-resolution in two-photon excitation (2PE) microscopy offers new approaches for visualizing the deep inside the brain functions at the nanoscale. In this study, we developed a novel 2PE stimulated-emission-depletion (STED) microscope with all-synchronized picosecond pulse light sources and time...

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Detalles Bibliográficos
Autores principales: Ishii, Hirokazu, Otomo, Kohei, Chang, Ching-Pu, Yamasaki, Miwako, Watanabe, Masahiko, Yokoyama, Hiroyuki, Nemoto, Tomomi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449175/
https://www.ncbi.nlm.nih.gov/pubmed/37616194
http://dx.doi.org/10.1371/journal.pone.0290550

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449175/
https://www.ncbi.nlm.nih.gov/pubmed/37616194
http://dx.doi.org/10.1371/journal.pone.0290550

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