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All-synchronized picosecond pulses and time-gated detection improve the spatial resolution of two-photon STED microscopy in brain tissue imaging
Super-resolution in two-photon excitation (2PE) microscopy offers new approaches for visualizing the deep inside the brain functions at the nanoscale. In this study, we developed a novel 2PE stimulated-emission-depletion (STED) microscope with all-synchronized picosecond pulse light sources and time...
Autores principales: | Ishii, Hirokazu, Otomo, Kohei, Chang, Ching-Pu, Yamasaki, Miwako, Watanabe, Masahiko, Yokoyama, Hiroyuki, Nemoto, Tomomi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449175/ https://www.ncbi.nlm.nih.gov/pubmed/37616194 http://dx.doi.org/10.1371/journal.pone.0290550 |
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