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A general method for the development of multicolor biosensors with large dynamic ranges

Fluorescent biosensors enable the study of cell physiology with spatiotemporal resolution; yet, most biosensors suffer from relatively low dynamic ranges. Here, we introduce a family of designed Förster resonance energy transfer (FRET) pairs with near-quantitative FRET efficiencies based on the reve...

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Detalles Bibliográficos
Autores principales: Hellweg, Lars, Edenhofer, Anna, Barck, Lucas, Huppertz, Magnus-Carsten, Frei, Michelle. S., Tarnawski, Miroslaw, Bergner, Andrea, Koch, Birgit, Johnsson, Kai, Hiblot, Julien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10449634/
https://www.ncbi.nlm.nih.gov/pubmed/37291200
http://dx.doi.org/10.1038/s41589-023-01350-1